Il Torneo di pallacanestro maschile NCAA Division I 2000 si concluse con la vittoria di Michigan State contro Florida.
Mateen Cleaves di Michigan State fu nominato Most Outstanding Player.
| Ottavi di finale | | | Quarti di finale | | | Sweet Sixteen | | | Elite Eight | |
| | | | | | | | | | | | | | | | | | | |
| 1 | Villanova | 87 | | | | |
|
| 16 | Liberty | 51 | |
| | 1 | Duke | 69 | |
| | |
| | | 8 | Kansas | 64 | |
| 8 | Oklahoma | 55 | |
| |
| 9 | BYU | 87 | |
| | 1 | Duke | 78 | |
| | |
| | | 5 | Florida | 87 | |
| 5 | Kansas State | 72 | | |
| |
| 12 | Murray State | 63 | |
| | 5 | Florida | 93 | |
| | |
| | | 4 | Illinois | 76 | |
| 4 | Arizona | 86 | |
| |
| 13 | North Carolina A&T | 71 | |
| | 5 | Florida | 77 | |
| | |
| | | 3 | Oklahoma State | 65 | |
| 6 | Arkansas | 91 | | | |
| |
| 11 | Saint Joseph's | 77 | |
| | 11 | Pepperdine | 67 | |
| | |
| | | 3 | Oklahoma State | 75 | |
| 3 | Duke | 102 | |
| |
| 14 | Valparaiso | 71 | |
| | 3 | Oklahoma State | 68 | |
| | |
| | | 10 | Seton Hall | 66 | |
| 7 | Iowa | 85 | | |
| |
| 10 | NC State | 72 | |
| | 10 | Seton Hall | 67 | |
| | |
| | | 2 | Temple | 65* | |
| 2 | Texas | 95 | |
| |
| 15 | Lafayette | 47 | |
| Ottavi di finale | | | Quarti di finale | | | Sweet Sixteen | | | Elite Eight | |
| | | | | | | | | | | | | | | | | | | |
| 1 | Stanford | 84 | | | | |
|
| 16 | South Carolina St | 65 | |
| | 1 | Stanford | 53 | |
| | |
| | | 8 | North Carolina | 60 | |
| 8 | North Carolina | 84 | |
| |
| 9 | Missouri | 70 | |
| | 8 | North Carolina | 74 | |
| | |
| | | 4 | Tennessee | 69 | |
| 5 | Connecticut | 75 | | |
| |
| 12 | Utah St | 67 | |
| | 5 | Connecticut | 51 | |
| | |
| | | 4 | Tennessee | 65 | |
| 4 | Tennessee | 63 | |
| |
| 13 | Louisiana-Lafayette | 58 | |
| | 8 | North Carolina | 59 | |
| | |
| | | 7 | Tulsa | 55 | |
| 6 | Miami-FL | 75 | | | |
| |
| 11 | Arkansas | 71 | |
| | 6 | Miami-FL | 75 | |
| | |
| | | 3 | Ohio St | 62 | |
| 3 | Ohio St | 87 | |
| |
| 14 | Appalachian St | 61 | |
| | 6 | Miami-FL | 71 | |
| | |
| | | 7 | Tulsa | 80 | |
| 7 | Tulsa | 89 | | |
| |
| 10 | UNLV | 62 | |
| | 7 | Tulsa | 69 | |
| | |
| | | 2 | Cincinnati | 61 | |
| 2 | Cincinnati | 64 | |
| |
| 15 | UNC-Wilmington | 47 | |
| Ottavi di finale | | | Quarti di finale | | | Sweet Sixteen | | | Elite Eight | |
| | | | | | | | | | | | | | | | | | | |
| 1 | Michigan State | 65 | | | | |
|
| 16 | Valparaiso | 38 | |
| | 1 | Michigan State | 73 | |
| | |
| | | 8 | Utah | 61 | |
| 8 | Utah | 48 | |
| |
| 9 | St. Louis | 45 | |
| | 1 | Michigan State | 75 | |
| | |
| | | 4 | Syracuse | 58 | |
| 5 | Kentucky | 85 | | |
| |
| 12 | St. Bonaventure | 80** | |
| | 5 | Kentucky | 50 | |
| | |
| | | 4 | Syracuse | 52 | |
| 4 | Syracuse | 79 | |
| |
| 13 | Samford | 65 | |
| | 1 | Michigan State | 75 | |
| | |
| | | 2 | Iowa State | 64 | |
| 6 | UCLA | 65 | | | |
| |
| 11 | Ball State | 57 | |
| | 6 | UCLA | 105 | |
| | |
| | | 3 | Maryland | 70 | |
| 3 | Maryland | 74 | |
| |
| 14 | Iona | 59 | |
| | 6 | UCLA | 56 | |
| | |
| | | 2 | Iowa State | 80 | |
| 7 | Auburn | 72 | | |
| |
| 10 | Creighton | 69 | |
| | 7 | Auburn | 60 | |
| | |
| | | 2 | Iowa State | 79 | |
| 2 | Iowa State | 88 | |
| |
| 15 | Central Connecticut St | 78 | |
| Ottavi di finale | | | Quarti di finale | | | Sweet Sixteen | | | Elite Eight | |
| | | | | | | | | | | | | | | | | | | |
| 1 | Arizona | 71 | | | | |
|
| 16 | Jackson St. | 47 | |
| | 1 | Arizona | 59 | |
| | |
| | | 8 | Wisconsin | 66 | |
| 8 | Wisconsin | 66 | |
| |
| 9 | Fresno St. | 56 | |
| | 8 | Wisconsin | 61 | |
| | |
| | | 4 | LSU | 48 | |
| 5 | Texas | 77 | | |
| |
| 12 | Indiana St. | 61 | |
| | 5 | Texas | 67 | |
| | |
| | | 4 | LSU | 72 | |
| 4 | LSU | 64 | |
| |
| 13 | Southeast Missouri St. | 61 | |
| | 8 | Wisconsin | 64 | |
| | |
| | | 6 | Purdue | 60 | |
| 6 | Purdue | 62 | | | |
| |
| 11 | Dayton | 61 | |
| | 6 | Purdue | 66 | |
| | |
| | | 3 | Oklahoma | 62 | |
| 3 | Oklahoma | 74 | |
| |
| 14 | Winthrop | 50 | |
| | 6 | Purdue | 75 | |
| | |
| | | 10 | Gonzaga | 66 | |
| 7 | Louisville | 66 | | |
| |
| 10 | Gonzaga | 77 | |
| | 10 | Gonzaga | 82 | |
| | |
| | | 2 | St John's | 76 | |
| 2 | St John's | 61 | |
| |
| 15 | Northern Arizona | 56 | |
| Semifinali nazionali | | | Finale nazionale | |
| | | | | | | | | |
| E5 | Florida | 71 | | |
|
| S8 | North Carolina | 59 | |
| | E5 | Florida | 76 | |
| | |
| | | M1 | Michigan State | 89 | |
| M1 | Michigan State | 53 | |
| |
| W8 | Wisconsin | 41 | |
- Allenatore: Tom Izzo
- Vice-allenatori: Stan Heath, Brian Gregory, Mike Garland