Codon usage bias

Codon usage bias in Physcomitrella patens

Codon usage bias refers to differences in the frequency of occurrence of synonymous codons in coding DNA. A codon is a series of three nucleotides (a triplet) that encodes a specific amino acid residue in a polypeptide chain or for the termination of translation (stop codons).

There are 64 different codons (61 codons encoding for amino acids and 3 stop codons) but only 20 different translated amino acids. The overabundance in the number of codons allows many amino acids to be encoded by more than one codon. Because of such redundancy it is said that the genetic code is degenerate. The genetic codes of different organisms are often biased towards using one of the several codons that encode the same amino acid over the others—that is, a greater frequency of one will be found than expected by chance. How such biases arise is a much debated area of molecular evolution. Codon usage tables detailing genomic codon usage bias for organisms in GenBank and RefSeq can be found in the HIVE-Codon Usage Tables (HIVE-CUTs) project,[1] which contains two distinct databases, CoCoPUTs and TissueCoCoPUTs. Together, these two databases provide comprehensive, up-to-date codon, codon pair and dinucleotide usage statistics for all organisms with available sequence information and 52 human tissues, respectively.[2][3]

It is generally acknowledged that codon biases reflect the contributions of 3 main factors, GC-biased gene conversion that favors GC-ending codons in diploid organisms, arrival biases reflecting mutational preferences (typically favoring AT-ending codons), and natural selection for codons that are favorable in regard to translation.[4] [5] [6] Optimal codons in fast-growing microorganisms, like Escherichia coli or Saccharomyces cerevisiae (baker's yeast), reflect the composition of their respective genomic transfer RNA (tRNA) pool.[7] It is thought that optimal codons help to achieve faster translation rates and high accuracy. As a result of these factors, translational selection is expected to be stronger in highly expressed genes, as is indeed the case for the above-mentioned organisms.[8][9] In other organisms that do not show high growing rates or that present small genomes, codon usage optimization is normally absent, and codon preferences are determined by the characteristic mutational biases seen in that particular genome. Examples of this are Homo sapiens (human) and Helicobacter pylori.[10][11] Organisms that show an intermediate level of codon usage optimization include Drosophila melanogaster (fruit fly), Caenorhabditis elegans (nematode worm), Strongylocentrotus purpuratus (sea urchin), and Arabidopsis thaliana (thale cress).[12] Several viral families (herpesvirus, lentivirus, papillomavirus, polyomavirus, adenovirus, and parvovirus) are known to encode structural proteins that display heavily skewed codon usage compared to the host cell. The suggestion has been made that these codon biases play a role in the temporal regulation of their late proteins.[13]

The nature of the codon usage-tRNA optimization has been fiercely debated. It is not clear whether codon usage drives tRNA evolution or vice versa. At least one mathematical model has been developed where both codon usage and tRNA expression co-evolve in feedback fashion (i.e., codons already present in high frequencies drive up the expression of their corresponding tRNAs, and tRNAs normally expressed at high levels drive up the frequency of their corresponding codons). However, this model does not seem to yet have experimental confirmation. Another problem is that the evolution of tRNA genes has been a very inactive area of research.[citation needed]

Contributing factors[edit]

Different factors have been proposed to be related to codon usage bias, including gene expression level (reflecting selection for optimizing the translation process by tRNA abundance), guanine-cytosine content (GC content, reflecting horizontal gene transfer or mutational bias), guanine-cytosine skew (GC skew, reflecting strand-specific mutational bias), amino acid conservation, protein hydropathy, transcriptional selection, RNA stability, optimal growth temperature, hypersaline adaptation, and dietary nitrogen.[14][15][16][17][18][19]

Evolutionary theories[edit]

Mutational bias versus selection[edit]

Although the mechanism of codon bias selection remains controversial, possible explanations for this bias fall into two general categories. One explanation revolves around the selectionist theory, in which codon bias contributes to the efficiency and/or accuracy of protein expression and therefore undergoes positive selection. The selectionist model also explains why more frequent codons are recognized by more abundant tRNA molecules, as well as the correlation between preferred codons, tRNA levels, and gene copy numbers. Although it has been shown that the rate of amino acid incorporation at more frequent codons occurs at a much higher rate than that of rare codons, the speed of translation has not been shown to be directly affected and therefore the bias towards more frequent codons may not be directly advantageous. However, the increase in translation elongation speed may still be indirectly advantageous by increasing the cellular concentration of free ribosomes and potentially the rate of initiation for messenger RNAs (mRNAs).[20]

The second explanation for codon usage can be explained by mutational bias, a theory which posits that codon bias exists because of nonrandomness in the mutational patterns. In other words, some codons can undergo more changes and therefore result in lower equilibrium frequencies, also known as “rare” codons. Different organisms also exhibit different mutational biases, and there is growing evidence that the level of genome-wide GC content is the most significant parameter in explaining codon bias differences between organisms. Additional studies have demonstrated that codon biases can be statistically predicted in prokaryotes using only intergenic sequences, arguing against the idea of selective forces on coding regions and further supporting the mutation bias model. However, this model alone cannot fully explain why preferred codons are recognized by more abundant tRNAs.[20]

Mutation-selection-drift balance model[edit]

To reconcile the evidence from both mutational pressures and selection, the prevailing hypothesis for codon bias can be explained by the mutation-selection-drift balance model. This hypothesis states that selection favors major codons over minor codons, but minor codons are able to persist due to mutation pressure and genetic drift. It also suggests that selection is generally weak, but that selection intensity scales to higher expression and more functional constraints of coding sequences.[20]

Consequences of codon composition[edit]

Effect on RNA secondary structure[edit]

Because secondary structure of the 5’ end of mRNA influences translational efficiency, synonymous changes at this region on the mRNA can result in profound effects on gene expression. Codon usage in noncoding DNA regions can therefore play a major role in RNA secondary structure and downstream protein expression, which can undergo further selective pressures. In particular, strong secondary structure at the ribosome-binding site or initiation codon can inhibit translation, and mRNA folding at the 5’ end generates a large amount of variation in protein levels.[21]

Effect on transcription or gene expression[edit]

Heterologous gene expression is used in many biotechnological applications, including protein production and metabolic engineering. Because tRNA pools vary between different organisms, the rate of transcription and translation of a particular coding sequence can be less efficient when placed in a non-native context. For an overexpressed transgene, the corresponding mRNA makes a large percent of total cellular RNA, and the presence of rare codons along the transcript can lead to inefficient use and depletion of ribosomes and ultimately reduce levels of heterologous protein production. In addition, the composition of the gene (e.g. the total number of rare codons and the presence of consecutive rare codons) may also affect translation accuracy.[22][23] However, using codons that are optimized for tRNA pools in a particular host to overexpress a heterologous gene may also cause amino acid starvation and alter the equilibrium of tRNA pools. This method of adjusting codons to match host tRNA abundances, called codon optimization, has traditionally been used for expression of a heterologous gene. However, new strategies for optimization of heterologous expression consider global nucleotide content such as local mRNA folding, codon pair bias, a codon ramp, codon harmonization or codon correlations.[24][25] With the number of nucleotide changes introduced, artificial gene synthesis is often necessary for the creation of such an optimized gene.

Specialized codon bias is further seen in some endogenous genes such as those involved in amino acid starvation. For example, amino acid biosynthetic enzymes preferentially use codons that are poorly adapted to normal tRNA abundances, but have codons that are adapted to tRNA pools under starvation conditions. Thus, codon usage can introduce an additional level of transcriptional regulation for appropriate gene expression under specific cellular conditions.[25]

Effect on speed of translation elongation[edit]

Generally speaking for highly expressed genes, translation elongation rates are faster along transcripts with higher codon adaptation to tRNA pools, and slower along transcripts with rare codons. This correlation between codon translation rates and cognate tRNA concentrations provides additional modulation of translation elongation rates, which can provide several advantages to the organism. Specifically, codon usage can allow for global regulation of these rates, and rare codons may contribute to the accuracy of translation at the expense of speed.[26]

Effect on protein folding[edit]

Protein folding in vivo is vectorial, such that the N-terminus of a protein exits the translating ribosome and becomes solvent-exposed before its more C-terminal regions. As a result, co-translational protein folding introduces several spatial and temporal constraints on the nascent polypeptide chain in its folding trajectory. Because mRNA translation rates are coupled to protein folding, and codon adaptation is linked to translation elongation, it has been hypothesized that manipulation at the sequence level may be an effective strategy to regulate or improve protein folding. Several studies have shown that pausing of translation as a result of local mRNA structure occurs for certain proteins, which may be necessary for proper folding. Furthermore, synonymous mutations have been shown to have significant consequences in the folding process of the nascent protein and can even change substrate specificity of enzymes. These studies suggest that codon usage influences the speed at which polypeptides emerge vectorially from the ribosome, which may further impact protein folding pathways throughout the available structural space.[26]

Methods of analysis[edit]

In the field of bioinformatics and computational biology, many statistical methods have been proposed and used to analyze codon usage bias.[27] Methods such as the 'frequency of optimal codons' (Fop),[28] the relative codon adaptation (RCA)[29] or the codon adaptation index (CAI)[30] are used to predict gene expression levels, while methods such as the 'effective number of codons' (Nc) and Shannon entropy from information theory are used to measure codon usage evenness.[31] Multivariate statistical methods, such as correspondence analysis and principal component analysis, are widely used to analyze variations in codon usage among genes.[32] There are many computer programs to implement the statistical analyses enumerated above, including CodonW, GCUA, INCA, etc. Codon optimization has applications in designing synthetic genes and DNA vaccines. Several software packages are available online for this purpose (refer to external links).[citation needed]

References[edit]

  1. ^ Athey, John; Alexaki, Aikaterini; Osipova, Ekaterina; Rostovtsev, Alexandre; Santana-Quintero, Luis V.; Katneni, Upendra; Simonyan, Vahan; Kimchi-Sarfaty, Chava (2017-09-02). "A new and updated resource for codon usage tables". BMC Bioinformatics. 18 (391): 391. doi:10.1186/s12859-017-1793-7. PMC 5581930. PMID 28865429.
  2. ^ Alexaki, Aikaterini; Kames, Jacob; Holcomb, David D.; Athey, John; Santana-Quintero, Luis V.; Lam, Phuc Vihn Nguyen; Hamasaki-Katagiri, Nobuko; Osipova, Ekaterina; Simonyan, Vahan; Bar, Haim; Komar, Anton A.; Kimchi-Sarfaty, Chava (June 2019). "Codon and Codon-Pair Usage Tables (CoCoPUTs): Facilitating Genetic Variation Analyses and Recombinant Gene Design". Journal of Molecular Biology. 431 (13): 2434–2441. doi:10.1016/j.jmb.2019.04.021. PMID 31029701. S2CID 139104807.
  3. ^ Kames, Jacob; Alexaki, Aikaterini; Holcomb, David D.; Santana-Quintero, Luis V.; Athey, John C.; Hamasaki-Katagiri, Nobuko; Katneni, Upendra; Golikov, Anton; Ibla, Juan C.; Bar, Haim; Kimchi-Sarfaty, Chava (January 2020). "TissueCoCoPUTs: Novel Human Tissue-Specific Codon and Codon-Pair Usage Tables Based on Differential Tissue Gene Expression". Journal of Molecular Biology. 432 (11): 3369–3378. doi:10.1016/j.jmb.2020.01.011. PMID 31982380.
  4. ^ P. Shah and M. A. Gilchrist (2011). "Explaining complex codon usage patterns with selection for translational efficiency, mutation bias, and genetic drift". Proceedings of the National Academy of Sciences of the United States of America. 108 (25): 10231–6. doi:10.1073/pnas.1016719108. PMC 3121864. PMID 21646514.
  5. ^ L. Duret and N. Galtier (2009). "Biased gene conversion and the evolution of mammalian genomic landscapes". Annu Rev Genomics Hum Genet. 10: 285–311. doi:10.1146/annurev-genom-082908-150001.
  6. ^ N. Galtier, C. Roux, M. Rousselle, J. Romiguier, E. Figuet, S. Glemin, N. Bierne and L. Duret (2018). "Codon Usage Bias in Animals: Disentangling the Effects of Natural Selection, Effective Population Size, and GC-Biased Gene Conversion". Mol Biol Evol. 35 (5): 1092–1103. doi:10.1093/molbev/msy015. hdl:20.500.12210/34500.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  7. ^ Dong, Hengjiang; Nilsson, Lars; Kurland, Charles G. (1996). "Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates". Journal of Molecular Biology. 260 (5): 649–663. doi:10.1006/jmbi.1996.0428. ISSN 0022-2836. PMID 8709146.
  8. ^ Sharp, Paul M.; Stenico, Michele; Peden, John F.; Lloyd, Andrew T. (1993). "Codon usage: mutational bias, translational selection, or both?". Biochem. Soc. Trans. 21 (4): 835–841. doi:10.1042/bst0210835. PMID 8132077. S2CID 8582630.
  9. ^ Kanaya, Shigehiko; Yamada, Yuko; Kudo, Yoshihiro; Ikemura, Toshimichi (1999). "Studies of codon usage and tRNA genes of 18 unicellular organisms and quantification of Bacillus subtilis tRNAs: gene expression level and species-specific diversity of codon usage based on multivariate analysis". Gene. 238 (1): 143–155. doi:10.1016/s0378-1119(99)00225-5. ISSN 0378-1119. PMID 10570992.
  10. ^ Atherton, John C.; Sharp, Paul M.; Lafay, Bénédicte (2000-04-01). "Absence of translationally selected synonymous codon usage bias in Helicobacter pylori". Microbiology. 146 (4): 851–860. doi:10.1099/00221287-146-4-851. ISSN 1350-0872. PMID 10784043.
  11. ^ Bornelöv, Susanne; Selmi, Tommaso; Flad, Sophia; Dietmann, Sabine; Frye, Michaela (2019-06-07). "Codon usage optimization in pluripotent embryonic stem cells". Genome Biology. 20 (1): 119. doi:10.1186/s13059-019-1726-z. ISSN 1474-760X. PMC 6555954. PMID 31174582.
  12. ^ Duret, Laurent (2000). "tRNA gene number and codon usage in the C. elegans genome are co-adapted for optimal translation of highly expressed genes". Trends in Genetics. 16 (7): 287–289. doi:10.1016/s0168-9525(00)02041-2. ISSN 0168-9525. PMID 10858656.
  13. ^ Shin, Young C.; Bischof, Georg F.; Lauer, William A.; Desrosiers, Ronald C. (2015-09-10). "Importance of codon usage for the temporal regulation of viral gene expression". Proceedings of the National Academy of Sciences. 112 (45): 14030–14035. Bibcode:2015PNAS..11214030S. doi:10.1073/pnas.1515387112. PMC 4653223. PMID 26504241.
  14. ^ Ermolaeva MD (October 2001). "Synonymous codon usage in bacteria". Curr Issues Mol Biol. 3 (4): 91–7. PMID 11719972.
  15. ^ Lynn DJ, Singer GA, Hickey DA (October 2002). "Synonymous codon usage is subject to selection in thermophilic bacteria". Nucleic Acids Res. 30 (19): 4272–7. doi:10.1093/nar/gkf546. PMC 140546. PMID 12364606.
  16. ^ Paul S, Bag SK, Das S, Harvill ET, Dutta C (2008). "Molecular signature of hypersaline adaptation: insights from genome and proteome composition of halophilic prokaryotes". Genome Biol. 9 (4): R70. doi:10.1186/gb-2008-9-4-r70. PMC 2643941. PMID 18397532.
  17. ^ Kober, K. M.; Pogson, G. H. (2013). "Genome-Wide Patterns of Codon Bias Are Shaped by Natural Selection in the Purple Sea Urchin, Strongylocentrotus purpuratus". G3. 3 (7): 1069–1083. doi:10.1534/g3.113.005769. PMC 3704236. PMID 23637123.
  18. ^ McInerney, James O. (1998-09-01). "Replicational and transcriptional selection on codon usage in Borrelia burgdorferi". Proceedings of the National Academy of Sciences. 95 (18): 10698–10703. Bibcode:1998PNAS...9510698M. doi:10.1073/pnas.95.18.10698. ISSN 0027-8424. PMC 27958. PMID 9724767.
  19. ^ Seward, Emily; Kelly, Steve (2016). "Dietary nitrogen alters codon bias and genome composition in parasitic microorganisms". Genome Biology. 17 (226): 3–15. doi:10.1186/s13059-016-1087-9. PMC 5109750. PMID 27842572.
  20. ^ a b c Hershberg, R; Petrov, D. A. (2008). "Selection on codon bias". Annual Review of Genetics. 42: 287–99. doi:10.1146/annurev.genet.42.110807.091442. PMID 18983258. S2CID 7085012.
  21. ^ Novoa, E. M.; Ribas De Pouplana, L (2012). "Speeding with control: Codon usage, tRNAs, and ribosomes". Trends in Genetics. 28 (11): 574–81. doi:10.1016/j.tig.2012.07.006. PMID 22921354.
  22. ^ Shu, P.; Dai, H.; Gao, W.; Goldman, E. (2006). "Inhibition of translation by consecutive rare leucine codons in E. coli: absence of effect of varying mRNA stability". Gene Expr. 13 (2): 97–106. doi:10.3727/000000006783991881. PMC 6032470. PMID 17017124.
  23. ^ Correddu, D.; Montaño López, J. d. J.; Angermayr, S. A.; Middleditch, M. J.; Payne, L. S.; Leung, I. K. H. (2019). "Effect of Consecutive Rare Codons on the Recombinant Production of Human Proteins in Escherichia coli". IUBMB Life. 72 (2): 266–274. doi:10.1002/iub.2162. hdl:11343/286411. PMID 31509345. S2CID 202555575.
  24. ^ Mignon, C.; Mariano, N.; Stadthagen, G.; Lugari, A.; Lagoutte, P.; Donnat, S.; Chenavas, S.; Perot, C.; Sodoyer, R.; Werle, B. (2018). "Codon harmonization - going beyond the speed limit for protein expression". FEBS Letters. 592 (9): 1554–1564. doi:10.1002/1873-3468.13046. PMID 29624661.
  25. ^ a b Plotkin, J. B.; Kudla, G (2011). "Synonymous but not the same: The causes and consequences of codon bias". Nature Reviews Genetics. 12 (1): 32–42. doi:10.1038/nrg2899. PMC 3074964. PMID 21102527.
  26. ^ a b Spencer, P. S.; Barral, J. M. (2012). "Genetic Code Redundancy and Its Influence on the Encoded Polypeptides". Computational and Structural Biotechnology Journal. 1: 1–8. doi:10.5936/csbj.201204006. PMC 3962081. PMID 24688635.
  27. ^ Comeron JM, Aguadé M (September 1998). "An evaluation of measures of synonymous codon usage bias". J. Mol. Evol. 47 (3): 268–74. Bibcode:1998JMolE..47..268C. doi:10.1007/PL00006384. PMID 9732453. S2CID 21862217.
  28. ^ Ikemura T (September 1981). "Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system". J. Mol. Biol. 151 (3): 389–409. doi:10.1016/0022-2836(81)90003-6. PMID 6175758.
  29. ^ Fox JM, Erill I (June 2010). "Relative codon adaptation: a generic codon bias index for prediction of gene expression". DNA Res. 17 (3): 185–96. doi:10.1093/dnares/dsq012. PMC 2885275. PMID 20453079.
  30. ^ Sharp, Paul M.; Li, Wen-Hsiung (1987). "The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications". Nucleic Acids Research. 15 (3): 1281–1295. doi:10.1093/nar/15.3.1281. PMC 340524. PMID 3547335.
  31. ^ Peden J (2005-04-15). "Codon usage indices". Correspondence Analysis of Codon Usage. SourceForge. Retrieved 2010-10-20.
  32. ^ Suzuki H, Brown CJ, Forney LJ, Top EM (December 2008). "Comparison of correspondence analysis methods for synonymous codon usage in bacteria". DNA Res. 15 (6): 357–65. doi:10.1093/dnares/dsn028. PMC 2608848. PMID 18940873.

External links[edit]

Retrieved from "https://en.wikipedia.org/w/index.php?title=Codon_usage_bias&oldid=1193970285"