• Thumbnail for Two-hybrid screening
    Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions...
    47 KB (6,112 words) - 03:07, 1 April 2024
  • Thumbnail for High-throughput screening
    Synthetic genetic array Virtual high throughput screening Yeast two-hybrid screening Zhao, Yicheng; Heumueller, Thomas; Zhang, Jiyun; Luo, Junsheng; Kasian...
    33 KB (4,120 words) - 21:18, 16 February 2024
  • Thumbnail for Protein–protein interaction
    most conventional and widely used high-throughput methods are yeast two-hybrid screening and affinity purification coupled to mass spectrometry. This system...
    74 KB (8,391 words) - 16:05, 7 April 2024
  • Thumbnail for Protein
    the relative levels of the various proteins present in a cell, and two-hybrid screening, which allows the systematic exploration of protein–protein interactions...
    87 KB (9,875 words) - 00:02, 21 April 2024
  • yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain is sold commercially by Clontech and is used as a partner...
    932 bytes (99 words) - 00:43, 6 July 2023
  • Thumbnail for GAL4/UAS system
    The GAL4/UAS system is also utilized in Two-Hybrid Screening, a method of identifying interactions between two proteins or a protein with DNA. Gal4 expression...
    13 KB (1,800 words) - 00:46, 20 September 2023
  • Thumbnail for Reporter gene
    expression. A more complex use of reporter genes on a large scale is in two-hybrid screening, which aims to identify proteins that natively interact with one...
    17 KB (1,980 words) - 11:31, 24 March 2024
  • calcium from the blood and depositing it, in insoluble form, in the bones. The two homeostatic mechanisms working through PTH on the one hand, and calcitonin...
    80 KB (9,585 words) - 18:05, 16 April 2024
  • yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain has been sold commercially by Clontech since at least...
    1 KB (167 words) - 01:12, 6 July 2023
  • transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of E. coli in which a desired protein-DNA or...
    2 KB (177 words) - 09:41, 30 January 2021