molecular biology, subcloning is a technique used to move a particular DNA sequence from a parent vector to a destination vector. Subcloning is not to be confused...

T cloning) is a subcloning technique that avoids the use of restriction enzymes and is easier and quicker than traditional subcloning. The technique relies...

strand. The concept is used in molecular biology, in cloning, or when subcloning insert DNA into vector DNA. Such ends may be generated by restriction...

One application for this practice is for use in TA cloning, a special subcloning technique similar to PCR, where efficiency can be increased by adding...

genomes. Cancers usually show tumour heterogeneity, containing multiple subclones. They also frequently have reduced expression of DNA repair enzymes due...

electrophoresis, and chromatography. It is used in genetic fingerprinting, plasmid subcloning, and RFLP analysis. A given restriction enzyme cuts DNA segments within...

reported in 1973. A neuroblast-like subclone of SK-N-SH, named SH-SY, was subcloned as SH-SY5, which was subcloned a third time to produce the SH-SY5Y...

ob/ob mice FBS+ I+ T3 TA1 Subclone of C3H10T1/2 FBS + D + I 30A5 Subclone of C3H10T1/2 FBS + D + M + I 1246 Adipogenic Subclone of CH3 mouse teratocarcinoma...

may give rise to subclones that possess an evolutionary advantage over the others within the tumour environment, and these subclones may become dominant...

the intensity, and high heterogeneity - variability - of tumor cell subclones. Phylogenetics can also aid in drug design and discovery. Phylogenetics...