and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism...
20 KB (2,517 words) - 08:10, 5 October 2023
molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation...
32 KB (4,016 words) - 17:56, 20 August 2024
found in a variety of vectors, including cloning vectors to increase the number of copies of target DNA, and in expression vectors to create a protein product...
5 KB (683 words) - 08:04, 18 May 2024
target cell. All vectors may be used for cloning and are therefore cloning vectors, but there are also vectors designed specially for cloning, while others...
18 KB (2,164 words) - 18:27, 4 June 2024
another cell Cloning vector, a small piece of DNA into which a foreign DNA fragment can be inserted for cloning purposes Shuttle vector, a plasmid constructed...
6 KB (733 words) - 12:35, 8 September 2024
Plasmid (redirect from Plasmid vector)
Microbiology Society. Brown TA (2010). "Chapter 2 – Vectors for Gene Cloning: Plasmids and Bacteriophages". Gene Cloning and DNA Analysis: An Introduction (6th ed...
46 KB (5,311 words) - 19:21, 7 September 2024
multiple cloning site. The cloned gene may be transferred from a specialized cloning vector to an expression vector, although it is possible to clone directly...
34 KB (4,178 words) - 01:07, 24 March 2024
notoriety for being the first mammal cloned from a somatic cell. Another example of artificial cloning is molecular cloning, a technique in molecular biology...
107 KB (11,492 words) - 17:05, 20 September 2024
pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. The designation "pUC" is derived from the classical "p"...
8 KB (865 words) - 02:56, 5 August 2024
Golden Gate Cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple...
18 KB (2,489 words) - 05:22, 30 April 2024