pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. The designation "pUC" is derived from the classical "p"...
8 KB (916 words) - 09:19, 18 May 2024
including EcoRI, BamHI, and PstI. Another vector used in genetic engineering is pUC19, which is similar to pUC18, but its polylinker region is reversed. E.coli...
5 KB (683 words) - 08:04, 18 May 2024
The plasmid must contain the lacZα, and examples of such plasmids are pUC19 and pBluescript. The E. coli cell should contain the mutant lacZ gene with...
13 KB (1,821 words) - 00:36, 3 December 2023
vectors are available. Many plasmids have high copy numbers, for example, pUC19 has a copy number of 500-700 copies per cell, and high copy number is useful...
20 KB (2,517 words) - 08:10, 5 October 2023
different strains of Bacillus subtilis and the Escherichia coli plasmid pUC19 were exposed to selected conditions of space (space vacuum and/or defined...
5 KB (542 words) - 23:04, 20 November 2023
transformation. A transformation efficiency of 1×108 cfu/μg for a small plasmid like pUC19 is roughly equivalent to 1 in 2000 molecules of the plasmid used being introduced...
23 KB (2,952 words) - 07:47, 19 May 2024
cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors". Gene. 33 (1): 103–19. doi:10.1016/0378-1119(85)90120-9. PMID 2985470...
13 KB (943 words) - 15:51, 13 March 2024
unsuccessful blue ones. Example of cloning vectors used for this test are pUC19, pBluescript, pGem-T Vectors, and it also requires the use of specific E...
11 KB (1,061 words) - 16:57, 16 October 2023
set of universal M13 primers. Three years later, the currently popular pUC19 plasmid was engineered by the same scientists. The gene on a DNA sequence...
14 KB (1,699 words) - 14:24, 8 July 2023
resistance to the antibiotic Geneticin. Modern bacterial cloning vectors (e.g. pUC19 and later derivatives including the pGEM vectors) use the blue-white screening...
32 KB (4,016 words) - 16:47, 6 April 2024