Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. The quantity...
22 KB (2,933 words) - 21:05, 2 May 2024
ELISA (redirect from Enzyme-linked immunosorbent assay)
The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzə/, /ˌiːˈlaɪzə/) is a commonly used analytical biochemistry assay, first described by Eva Engvall...
34 KB (4,392 words) - 19:57, 5 May 2024
amount of the enzyme that catalyzes the conversion of one micromole of substrate per minute under the specified conditions of the assay method. The specified...
3 KB (397 words) - 02:47, 20 April 2024
density, functional activity in enzyme international units, degree of effect in comparison to a standard, etc.). If the assay involves exogenous reactants...
28 KB (3,322 words) - 17:17, 24 December 2023
product. Occasionally, an assay fails and approaches are essential to resurrect a failed assay. The most sensitive enzyme assays use lasers focused through...
71 KB (9,346 words) - 09:46, 1 June 2024
needed] The diagnosis may be confirmed by enzyme assay of skin fibroblasts and white blood cells. The enzyme assay is considered to be the most credible diagnostic...
29 KB (3,059 words) - 11:40, 8 June 2024
Reporter gene (redirect from Reporter gene assay)
Reporter genes can be used to assay for the expression of a gene of interest that is normally difficult to quantitatively assay. Reporter genes can produce...
17 KB (1,980 words) - 11:31, 24 March 2024
genetic risks. Enzyme assay techniques detect individuals with lower levels of hexosaminidase A. Development of a serum enzyme assay test made it feasible...
19 KB (2,532 words) - 14:40, 27 October 2023
commonly obtained from enzyme assays. In 1913 Leonor Michaelis and Maud Leonora Menten proposed a quantitative theory of enzyme kinetics, which is referred...
96 KB (9,819 words) - 08:12, 3 June 2024
The MTT assay is a colorimetric assay for assessing cell metabolic activity. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions...
13 KB (1,522 words) - 20:50, 18 May 2024